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negative control mutated tcf/lef binding site (fopflash  (Upstate Biotechnology Inc)
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Effects of Siah1 on <t>Tcf/Lef</t> regulated transcription activity in SKBR3 and MCF-7 cells . A: Transfected cells were cotransfected with Topflash or <t>Fopflash</t> plasmid, the Renilla luciferase reporter plasmid (pRL-TK) as an internal control and the indicated expression plasmids. Luciferase activity was measured at 24 h after transfection and plotted after normalizing with respect to the Renilla luciferase activity. Each experiment was performed at least three times. Columns, mean; bars, SD; *, p < 0.05. B; Functional inhibition of Siah-1 ubiquitin-ligase by siRNA increased TCF/Lef transcriptional activity in MCF-7 cells at 24 hours after transfection. Each experiment was performed three times. Columns, mean; bars, SD; *, Siah1 siRNA vs. both untreated and control siRNA p < 0.05.
Negative Control Mutated Tcf/Lef Binding Site (Fopflash, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+mutated+tcf/negative+control+mutated+tcf+lef+binding+site++fopflash/pmc02921397-42-11-29
Average 90 stars, based on 1 article reviews
negative control mutated tcf/lef binding site (fopflash - by Bioz Stars, 2026-10
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fop-mutated tcf/lef negative control  (Addgene inc)
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Effects of Siah1 on <t>Tcf/Lef</t> regulated transcription activity in SKBR3 and MCF-7 cells . A: Transfected cells were cotransfected with Topflash or <t>Fopflash</t> plasmid, the Renilla luciferase reporter plasmid (pRL-TK) as an internal control and the indicated expression plasmids. Luciferase activity was measured at 24 h after transfection and plotted after normalizing with respect to the Renilla luciferase activity. Each experiment was performed at least three times. Columns, mean; bars, SD; *, p < 0.05. B; Functional inhibition of Siah-1 ubiquitin-ligase by siRNA increased TCF/Lef transcriptional activity in MCF-7 cells at 24 hours after transfection. Each experiment was performed three times. Columns, mean; bars, SD; *, Siah1 siRNA vs. both untreated and control siRNA p < 0.05.
Fop Mutated Tcf/Lef Negative Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+mutated+tcf/fop+mutated+tcf+lef+negative+control/pm30926792-281-20-23
Average 90 stars, based on 1 article reviews
fop-mutated tcf/lef negative control - by Bioz Stars, 2026-10
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pgl3-of (negative control with mutated tcf/lef binding site)  (Promega)
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Effects of Siah1 on <t>Tcf/Lef</t> regulated transcription activity in SKBR3 and MCF-7 cells . A: Transfected cells were cotransfected with Topflash or <t>Fopflash</t> plasmid, the Renilla luciferase reporter plasmid (pRL-TK) as an internal control and the indicated expression plasmids. Luciferase activity was measured at 24 h after transfection and plotted after normalizing with respect to the Renilla luciferase activity. Each experiment was performed at least three times. Columns, mean; bars, SD; *, p < 0.05. B; Functional inhibition of Siah-1 ubiquitin-ligase by siRNA increased TCF/Lef transcriptional activity in MCF-7 cells at 24 hours after transfection. Each experiment was performed three times. Columns, mean; bars, SD; *, Siah1 siRNA vs. both untreated and control siRNA p < 0.05.
Pgl3 Of (Negative Control With Mutated Tcf/Lef Binding Site), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/negative+control+mutated+tcf/pgl3+basic/pm22296502-83-15-32
Average 90 stars, based on 1 article reviews
pgl3-of (negative control with mutated tcf/lef binding site) - by Bioz Stars, 2026-10
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Effects of Siah1 on Tcf/Lef regulated transcription activity in SKBR3 and MCF-7 cells . A: Transfected cells were cotransfected with Topflash or Fopflash plasmid, the Renilla luciferase reporter plasmid (pRL-TK) as an internal control and the indicated expression plasmids. Luciferase activity was measured at 24 h after transfection and plotted after normalizing with respect to the Renilla luciferase activity. Each experiment was performed at least three times. Columns, mean; bars, SD; *, p < 0.05. B; Functional inhibition of Siah-1 ubiquitin-ligase by siRNA increased TCF/Lef transcriptional activity in MCF-7 cells at 24 hours after transfection. Each experiment was performed three times. Columns, mean; bars, SD; *, Siah1 siRNA vs. both untreated and control siRNA p < 0.05.

Journal: BMC Cancer

Article Title: Siah1 proteins enhance radiosensitivity of human breast cancer cells

doi: 10.1186/1471-2407-10-403

Figure Lengend Snippet: Effects of Siah1 on Tcf/Lef regulated transcription activity in SKBR3 and MCF-7 cells . A: Transfected cells were cotransfected with Topflash or Fopflash plasmid, the Renilla luciferase reporter plasmid (pRL-TK) as an internal control and the indicated expression plasmids. Luciferase activity was measured at 24 h after transfection and plotted after normalizing with respect to the Renilla luciferase activity. Each experiment was performed at least three times. Columns, mean; bars, SD; *, p < 0.05. B; Functional inhibition of Siah-1 ubiquitin-ligase by siRNA increased TCF/Lef transcriptional activity in MCF-7 cells at 24 hours after transfection. Each experiment was performed three times. Columns, mean; bars, SD; *, Siah1 siRNA vs. both untreated and control siRNA p < 0.05.

Article Snippet: The Tcf/Lef-responsive luciferase reporter gene (Topflash), the negative control with mutated Tcf/Lef binding site (Fopflash), and the Renilla luciferase reporter plasmid (pRL-TK) as an internal control were obtained from Upstate Biotechnology, USA.

Techniques: Activity Assay, Transfection, Plasmid Preparation, Luciferase, Expressing, Functional Assay, Inhibition